Company Information

• Name: Intelligence & Technology Lab. Inc. (I Tech Lab Inc.)
• Establishment: August 2011
• Representative: Shin Nakamura, PhD, President
• Capital: 21 million yen
• Employees: 11 (PhD:3, Veterinarians :2+(1), Pharmacists:2)
• Address & Contact: 52-1 Fukue, Kizu-cho, Kizu, Gifu 503-0628, Japan
  Tel: 81-584-54-0015, FAX:81-584-54-0017
  E-mail: itl@itechlab.co.jp
  URL http://itechlab.co.jp
• Partnership: Comprehensive collaboration with NPO Primate Agora (URL: http://primate-agora.jp )
• Mission: I Tech Lab is a new type of CRO that uses non-human primates, genomics/transcriptomics, advanced technology, and pharmacologic techniques in research to provide highly reliable results at reasonable costs. These researches are performed through intelligence and original ideas accumulated through past experience and achievements. We aim to develop collaborative tests while also carrying out R&D roles which are not found in conventional CROs.

Studies Using Monkey Models (Cynomolgus, Rhesus, and/or Japanese Macaques) and Genomics/Transcriptomics Analysis

Studies in Monkeys

• Studies on drug efficacy for allergic disorder using macaque model (typeⅠ, type Ⅲ and type Ⅳ allergy), especially in pollinosis and its related disorders.
• Studies on functional and molecular mechanisms of drug, traditional medicine, functional food, cosmetics and/or their materials using usual animal models using Genomics/Transcriptomics with bioinformatics.

Studies on Monkey B-Virus and SRV

• HVP2-ELISA for B-virus monitoring by sero-test.
• Genomic diagnosis for B-virus and discrimination from other related herpes virus.
• Diagnosis for SRV-4 & -5 is now on-going.

Other Genomics/Transcriptmics Studies

• Gene expression profiling of cytokines, hormones and other functional factors with q-RT-PCR and/or DNA microarray.
• Genomics studies on Intestinal flora, bacteria DNA quantitation by the q-PCR.
• Genomics studies on drug metabolism genes (CYPs) (q-RT-PCR, DNA microarray).
• Other genomics/transcriptomics analyses for samples from human, mouse, rat, and others.
• Studies on function and toxicity of drugs, foods and/or cosmetics using in-vitro cellular system.
• Cell-free studies on metabolomics, biochemical, gene expression, and/or related analysis.

Studies on Pharmacologic Effect or Toxicity Using Mouse and/or Rat

Pharmacologic works studies

• Memory and learning functional improvement testing (Mouse, Rat).
• Anti-obesity testing (Mouse, Rat).
• Anti-hyperlipidemic testing (Mouse, Rat, Rabbit).
• Tests of blood glucose.
• Whitening tests (Mouse, Guinea pig).
• Cerebrovascular disorder improvement tests.
• Circulatory functional improvement tests.
• Anti-inflammation and anti-allergic tests.
• Kidney disorder improvement tests.
• Drug efficacy tests on liver function.
• Anti-anxiety tests.
• Anti-depressive testing.
• Drug efficacy tests on digestive organs.
• Analgestic (antinociceptive) testing.
• Anti-oxidant testing.
• Anti-cancer testing.
• Evasion test using common mosquitoes.

Other available test models

• Collagenese induced intracerebral hemorrhage model (rat).
• Conditional place preference behavior tests (CPP test) (rat).
• Skin incision and skin defect tests (rat).
• Anti-fatigue test (forced swimming) (sleep deprivation model) (rat).
• Experimental allergic-neuritis model (rat).
• Retina-ischemia tests (rat).
• Hair restoration tests (mouse).
• Ear edema model (mouse).
• Anti-Meniere disease tests (endolymphatic hydrops model) (guinea pig).
• Topical anesthesia and infiltration anesthesia test (guinea pig).
• Dry eye model tests (rabbit).
• Intraocular pressure measurement tests (rat, rabbit).
• Histamine tests (cats).

Rental Lab

• Room space for animal care and/or/study and for experimental works.

Publications (recent ten years)

• Fusako Mitsunaga & Shin Nakamura: A Sensitive and Simple Method to Assess NK Cell Activity by RT-qPCR for Granzyme B Using Spleen and Blood, Journal of Biosciences and Medicines, 9, 27-38 (2021).
• Shin Nakamura：”Neutrophils Tissue Factor” Review, Japanese Journal of Thrombosis and Hemostasis, 29(1), 35-40 (2018) (in Japanese).
• Shin Nakamura and Fusako Mitsunaga: Anti-allergic Effect of Para-probiotics From Non-viable Acetic Acid Bacteria in Ovalbumin-sensitized Mice, Food and Nutrition Sciences, 9: 1376-1385 (2018) .
• Fusako Mitsunaga, Shin Nakamura, Motofumi Ohki: Practical use of Acoustic Rhinometry for Quantitative Assessment of Nasal Obstruction in an Allergic Model Using Macaque Monkey. Journal of Biosciences and Medicines,.5: (2), 1125-32 (2017).
• Fusako Mitsunaga, Masakazu Umezawa, Ken Takeda, Shin Nakamura：Maternal administration of nanomaterials elicits hemoglobin upregulation in the neonatal brain of non-human primates. The Journal of Toxicological Sciences 41-2: 265-271 (2016).
• Shigeo Nakayama, Fusako Mitsunaga, Akira Maeda, Shin Nakamura：Effect of a Natural Inulin-containing Product, "Kikuimo Extract” on Intestinal Microbes and Gene Expression in the Liver an Adipose Tissue of Menopausal Monkeys. Food and Nutrition Sciences 7: 752-762 (2016).
• Masaki Shintani, Fusako Mitsunaga, Shun Shimabukuro, Shin Nakamura：Anti-Inflammatory and Immunostimulatory Effects of Extract from Culture of Effective Microorganisms (ECEM) Revealed by Functional Genomics and Metabolome Analyses . Food and Nutrition Sciences 6: 1115-1125 (2015).
• Soo-Wan Chae, Fusako Mitsunaga, Su-Jin Jung, Ki-Chan Ha, Hong-Sig Sin, Seung-Hwan Jang, Shin Nakamura： Mechanisms Underlying the Antifatigue Effects of the Mycelium Extract of Cordyceps (Paecilomyces hepiali, CBG-CS-2) in Mice in the Forced Swimming Test. Food and Nutrition Sciences 6: 287-298 (2015).
• Soo-Wan Chae, Fusako Mitsunaga, Su-Jin Jung, Ki-Chan Ha, Hong-Sig Sin, Seung-Hwan Jang, Shin Nakamura：Nutrigenomic Study on Immunomodulatory Function of Cordyceps Mycelium Extract (Paecilomyces hepiali) in Mitomycin C–Treated Mice. Food and Nutrition Sciences 6: 2217-2224 (2014).
• A Takenaka1, S Nakamura, F Mitsunaga, M- I Murayama, TUdono, B Suryobroto 2012. Human-Specific SNP in Obesity Genes, Adrenergic Receptor Beta2 (ADRB2), Beta3 (ADRB3), and PPAR c2(PPARG), during Primate Evolution PLOS ONE 7 (8) e43461 (2012). em. the 4th AFLAS Congress Meeting (Nov. 2010, Taipei).
• Nakamura S, Mitsunaga F, Ueiwa M, Sugamata M, Takeda K. 2010. Effect of Nanomaterials to Immune System in Monkeys: Potent Induction of Alternatively Activated Macrophages. 14th International Congress of Immunology (Aug. 2010, Kobe).
• Nakamura S, Mitsunaga F, Goto H. 2010. Genomics Study on Action of an Oriental Medicine, TokiShakuyaku-San, Using Monkey Model. The 15th International congress of Oriental Medicine (Feb. 2010, Chiba)
• Mitsunaga F, Ueiwa M, Kamanaka Y, Morimoto M, Nakamura S. 2010. Fetal sex determination of macaque monkeys by a nested PCR using maternal plasma. Exp. Anim. 59: 255-260.